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KMID : 0387420100210030087
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Korean Society of Oriental Neuropsychiatry
2010 Volume.21 No. 3 p.87 ~ p.93
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Up-and-Down Procedure(UDP) Determinations of Acute Oral Toxicity of LMK02-Jangwonhwan in SD Rats
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Kang Hyung-Won
Kwon Yeong-Mi
Lee Sang-Won
Kim Ji-Hwon
Lee Hyo-Gyung
Jang Hyun-Ho
Park Bo-Ra
Lyu Yeoung-Su
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Abstract
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Objectives: The oriental medicine Jangwonhwan originally described in the Korean medical text, DonguiBogam(amnesia chapter). Recently, a modified formula of Jangwonhwan(LMK02-Jangwonhwan), was shown to reduce -amyloid deposition in the brain of Tg-APPswe/PS1dE9 mouse model for Alzheimer’s disease. This experiment aimed to investigate the acute oral toxicity of LMK02 in SD rats by up-and-down procedure determinations.
Methods: Quality control of tablet form of LMK02 was established by estimating indicative components, Ginsenoside Rg3 of Red Ginseng and Decursin of Angelicagigas Nakai. The toxicity of LMK02 was investigated in 6 week old, specific pathogen free(SPF), Sprageu-Dawley rats. 3 female rats received 5,000 mg/10 ml/kg of test substance and their death rate, clinical sings, weight changes and autopsy findings had been observed for 2 weeks.
Results: Any specific symptoms or death were resulted in this experiment. No significant changes in rats’ weight. No significant differences in atopsy.
Conslusions: The minimum lethal dose(MLD) of LMK02 for female Sprauge-Dawley rats were more than 5,000mg/kg in this experiment.
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KEYWORD
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Oriental medicine, Alzheimer disease, LMK02-Jangwonhwan, Toxicity test
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